An antibiotic-antimycotic cocktail is a combination of antibiotics and antifungal agents used to target multiple aspects of microbial growth in cell cultures. This cocktail typically includes antibiotics that target the cell wall to prevent the growth of bacteria; and antifungal agents to inhibit the growth of fungi. It also includes agents that target protein synthesis pathways which interferes with the production of bacterial proteins and which prevents the production of bacterial enzymes. This cocktail is used to reduce the risk of contamination in cell cultures, and to ensure optimal growth and health of the cells.